Determination of Orotic acid in milk
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چکیده
Method VFD0134J_E HPLC Column: Eurokat H, 10 μm, 300 x 8 mm Order No. 30GX340EKN Phase: Eurokat H, 10 μm Eluent: 0.01 N Sulfuric acid Gradient: Isocratic Flow rate: 0.6 ml/min Temperature: 60 °C Volume: 20 μL (Standard) Detection: UV, 210 nm Substances: Orotic acid, Lactic acid and Acetic acid
منابع مشابه
Hematologic responses in pernicious anemia to orotic acid.
By R. WAYNE RUNDLES AND SPENCER S. BREwER, JR. OROTIC ACID has become a compound of considerable metabolic interest in recent years since it appears to be a key intermediate in the synthesis of pyrimidines. It was discovered in the whey of cow’s milk by Biscara and Belloni in 1905, but its correct chemical structure was not established unfil 1930 by Bachstez.’2 Orotic acid has been isolated fro...
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Orotic acid, first discovered in ruminant milk, is an intermediate in the pyrimidine biosynthesis pathway of animal cells. Its synthesis is initiated by the formation of carbamoyl phosphate (CP) in the cytoplasm, with ammonia derived from glutamine. Ureotelic species also form CP in the first step of urea synthesis in liver mitochondria. For that, ammonia is derived from tissue fluid. When ther...
متن کاملQuantification of non-protein nitrogen components of infant formulae and follow-up milks: comparison with cows' and human milk.
The composition of fourteen infant formulae and six follow-up milks with regard to their free amino acids (including taurine), free nucleotides, orotic acid, and free and total l-carnitine content was studied. The levels found were compared with the limits established in European legislation and with the composition of human and cows' milk samples. HPLC methodologies, optimized and validated fo...
متن کاملDetermination of orotic acid in whey and modified whey products.
The concentration of orotic acid in dry whey ranged between 64 and 146 mg/100 g and appeared dependent on whey type. The orotic acid of modified whey products, prepared commercially by electrodialysis, ultrafiltration, gel filtration, or polyphosphate precipitation was between 7 and 124 mg/100 g, which was less than that of dry whey. It appears that whey processing methods do not concentrate or...
متن کاملStudies on Chemical Protection against Radiation. I. the Protective Effect of Orotic Acid against X-radiation Death in Mice.
An experiment was carried out to determine the protective effect of orotic acid (uracil-4-carboxylic acid) against X-rays. Orotic acid in dose of 0.05, 0.1, 1.0, 1.7 and 10 mg per mouse was given intra peritoneally to mice before or after a single dose whole-body X irradiation of 700 r or 550 r, after which the 30-day survival rate was observed. As a result it was concluded that it would be dif...
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